Advansta fluorescent western blot detection and western blotting...

Fluorescent Western blotting

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AdvanBlock-PF blocking solution

Non-protein blocking solution for fluorescent and chemiluminescent Western blotting

Low background - Protein-free block buffer for lower background with certain antibodies
Optimized - Stabilizes fluorescence of WesternBright MCF fluorescent secondary antibodies
Versatile - Compatible with fluorescent and chemiluminescent detection

WesternBright MCF

Multi-color fluorescent Western blotting in one kit

Try multicolor fluorescent western blotting today. Our kit includes everything except primary antibodies.

Multiplexing – visualize two proteins simultaneously!
Sensitivity –10x greater sensitivity than Cy dyes; pictogram detection
Quality Reagents – includes AdvanBlock™-PF blocking and AdvanWash /AdvanWash -IR for washing
Fast results – 3.5 hours for entire protocol
Compatible with imaging systems that detect Cy3 and Cy5 (MCF) or near-IR imaging systems (MCF-IR)
Fluorescent Detection – choose between visible (APC and RPE) or near IR (IR700 and IR800) kits with SpectraDye™ secondary antibodies

Kit Includes:
Secondary antibodies
Blocking solution
Washing solution
Pre-cut low-autofluorescence PVDF membranes
Background quenching sheets

SpectraDye Antibody Labeling Kits

Make your own fluorescent antibody in one easy step.

Quick and easy – one-step labeling in 30 minutes
Versatile - Labeled antibodies are compatible with immunofluorescence microscopy, flow cytometry, Western blotting
Flexible - choose from 6 commonly used fluorescent dyes
Save time and money – no secondary or extra steps, once you label your primary
Save antibody – labeling reaction requires as little as 10 µg antibody

Kit includes:
Antibody Labeling Buffer
SpectraDye Dye Solution*
Quenching Solution
Neutralization Buffer

Sufficient reagents to label up to 1 mg of antibody
*Except kit K-11060-010

Ready-to-image three-color fluorescent blot

Fluorescently labeled primary and secondary antibodies fluoresce in the red, green, and blue fluorescent channels (like Cy2, Cy3, and Cy5 equivalent dyes)
Convenient – an easy way to evaluate the performance of your fluorescence imaging system
Reliable – quality controlled, with lot-specific statistics provided for every blot

Fluorescent Western blotting