Fluorescent Western blotting

Transfer membranes
Transfer membranes

Convenient nitrocellulose and PVDF membranes for Western blotting

  • Performance – quality controlled for background-free Western blots
  • Convenience – membranes available pre-cut and as rolls
  • High signal-to-noise ratio – low background signal for clearer results
  • Flexibility – choose the right membrane for your Western blotting application

 

Membrane Options:

WesternBright PVDF-FL: fluorescent detection
WesternBright PVDF-CL: chemiluminescent detection; best stability for stripping and re-probing chemiluminescent blots
PVDF membranes available as rolls or pre-cut (7x9 cm, 10x15 cm, or 13x18 cm)

WesternBright NC: all-purpose nitrocellulose membrane
Nitrocellulose membranes available as rolls or pre-cut (8x10 cm)

Background Quenching Sheets
Background Quenching Sheets

An added layer to decrease background
For improved imaging of blots and gels

  • Better images by removing background fluorescence and stray light
  • Improved signal to noise ratios
  • Simple – just place sheet under the blot or gel
  • Compatible with chemiluminescent and fluorescent Western blots
  • Compatible with DNA gels and protein stains - including SYBR green, SYBR Gold, ethidium bromide, SYPRO® Orange, SYPRO Ruby and more

 

Development folders
Development folders

Designed specifically for blot handling and imaging, these clear plastic folders eliminate the frustrations of traditional wrap while improving consistency and clarity

  • Wrinkle-free, dust-free imaging – Avoid the wrinkling and dust that cause artifacts in imaging
  • Safe handling – No pressure damage or scratching of the membrane
  • Suitable for all blots – Image chemiluminescent and fluorescent blots
  • Convenient storage – Easily image and store dust-free, up to two blots in each clear folder
Blotting papers
Blotting papers

Pre-cut paper for gel to membrane electrophoretic transfers

  • Convenient – pre-cut to save time; sized to work with most minigel transfer assemblies
  • Standardized – uniform thickness for reproducible transfer assembly
  • Tested – confirmed to perform consistently in protein transfers without introducing artifacts or background noise
Incubation trays
Incubation trays

Convenient containers for staining and washing gels and membranes

  • Smooth interior – protects membranes and gels from scratches
  • Convenient – multiple sizes (5x7, 6x9, 9x11,10.5x15.5 cm) and designs available for different gels and blots
  • Attached lid – to protect experiments from dust or debris that can cause speckles on blot images
  • Save – minimize antibody and buffer usage by using appropriately sized containers
  • Multi-chamber trays – process 6 mini-blots simultaneously, each chamber requires as little as 3 mL of solution