Advansta fluorescent western blot detection and western blotting...

Fluorescent Western blotting

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Ready-to-image three-color fluorescent blot

Fluorescently labeled primary and secondary antibodies fluoresce in the red, green, and blue fluorescent channels (like Cy2, Cy3, and Cy5 equivalent dyes)
Convenient – an easy way to evaluate the performance of your fluorescence imaging system
Reliable – quality controlled, with lot-specific statistics provided for every blot

Rapid fluorescent stain for total protein normalization

Convenient – simple protocol that takes less than 20 minutes, no de-staining required
Versatile – compatible with chemiluminescent and IR700/IR800 fluorescent Western blotting
Accurate – linear across a broad range from 1-50μg of lysate, ideal for total protein normalization

FLASHBlot-SD transfer buffer

Rapid high efficiency semi-dry transfer buffer

Fast - high ionic strength formulation allows for protein transfer in 3 to 10 minutes when used with a compatible high current semi-dry system
Compatible - use your existing high current semi-dry transfer apparatus
Reproducible - consistent transfer across entire blot
Versatile - use nitrocellulose or PVDF membranes to achieve transfers with low background and high sensitivity with both chemiluminescent and fluorescent Western blots

LightSaver™ Fluorescence Enhancing Solution

Fluorescent Western blotting