AdvanStain Iris

AdvanStain Iris is a rapid and sensitive alternative to Ponceau S stain for protein detection on Nitrocellulose and PVDF membranes after transfer from polyacrylamide gels. The dark blue color provides a high contrast which facilitates the acquisition of visible images. The dye may also be imaged with epi blue light.

Flexible imaging, no destaining required

Figure 1. AdvanStain Iris demonstrates high sensitivity staining of HeLa lysate, no destaining is required prior to analysis by Western blot. AdvanStain Iris stain was applied to a nitrocellulose membrane for 10 minutes. (a) EPI Blue image (b) Visible image. Protein bands were observed in the 80ng lysate load. (c) After Staining, the membrane was blocked then probed with mouse anti-Vinculin (Boster #MA1103) followed by Goat anti-Mouse HRP (Advansta #R-05071-500). The Western blot was developed with WesternBright™ ECL Substrate (Advansta #K-12045).

Maximum sensitivity

Figure 2. High sensitivity stain detects as little as 2 ng of purified protein per band. Dilutions of purified transferrin protein were electrophoresed using SDS-PAGE and the protein was transferred to a nitrocellulose membrane then stained with AdvanStain Iris for 10 minutes. Image was acquired with EPI Blue light.

Saves time and outperforms Ponceau S

Figure 3. AdvanStain Iris is significantly more sensitive when compared to Ponceau S and compatible with PVDF membranes.Dilutions of purified BSA protein were electrophoresed using SDS-PAGE and the protein was transferred to a nitrocellulose or PVDF membrane then stained with AdvanStain Iris for 10 minutes or Ponceau S for 5 minutes after a 10 minute water rinse. Images were acquired with visible light.

Re-use up to 3X

Figure 4. AdvanStain Iris may be re-used three times.Dilutions of HeLa Lysate were electrophoresed using SDS-PAGE and the protein was transferred to a nitrocellulose membrane then stained with AdvanStain Iris for 10 minutes. The staining solution was re-used three times to generate comparable data. Images were acquired with visible light.

• Advanstain Iris Brochure
• AdvanStain Iris short protocol
• AdvanStain Iris SDS
• Total Protein Stain Selection Guide
• Advansta’s Step-by-Step Guide to Western Blots