Protein staining

AdvanCell in-cell Western Kit
AdvanCell in-cell Western Kit

Total cell normalization and simultaneous detection of two biomarkers in whole cells

  • Save time – cells are directly probed, no need for time consuming lysate preparation
  • Quantitative immunofluorescence – normalize to cell number, allowing for accurate quantification and comparison of protein expression between wells
  • Detection of high and low molecular weight targets – a limitation of traditional Western blots is eliminated as high and low MW targets can easily be evaluated simultaneously
  • Detect proteins in situ in the most relevant cellular context with minimal sample processing
AdvanStain Iris
AdvanStain Iris

Upgrade your protein staining! Boost sensitivity,
no-destaining, and high-contrast.

  • Detection limit – 2 ng vs Ponceau at 150 ng
  • Quantitative – effective for lower protein concentrations
  • No destaining – allows direct chemiluminescent or fluorescent Western blotting without the need for destaining
  • Versatile – compatible with both nitrocellulose and PVDF membranes

 

Get a Free Sample

 

AdvanStain Total Fluorescent Protein Staining Kits
AdvanStain Total Fluorescent Protein Staining Kits

Rapid fluorescent stain for total protein normalization

 

  • Convenient – simple protocol that takes less than 20 minutes, no de-staining required
  • Versatile – compatible with chemiluminescent and IR700/IR800 fluorescent Western blotting
  • Accurate – linear across a broad range from 1-50μg of lysate, ideal for total protein normalization

Visio
Visio

Sample-loading buffer and protein stain in one

  • Instant results – see protein bands immediately as electrophoresis proceeds
  • Sensitive – detect as little as 10 to 25 ng per band
  • Fast protocol – no different from standard electrophoresis protocols - replace your current loading dye with Visio and samples labeled and ready to load after heating for 10 minutes
  • Flexible – use with reducing and non-reducing gels
  • Compatible – can be used with downstream mass spectrometry

 

WesternEaze-Chemi Kit
WesternEaze-Chemi Kit

Rapid, high sensitivity chemiluminescent Western blot kit for protein detection

 

  • Rapid – complete Western blot in 1 hour
  • Reproducible – fewer hands-on manipulations equate to consistent data
  • Convenient – contains all critical reagents required to perform a chemiluminescent Western
  • Versatile – kits are available for both mouse and rabbit primary antibodies

AdvanStain Ponceau
AdvanStain Ponceau

Protein stain for membranes

 

  • Quick – detect proteins in 5 minutes or less
  • Reversible – destain in minutes
  • Compatible – after destaining, PVDF and nitrocellulose membranes are ready for Western blotting