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Protein staining

AdvanCell in-cell Western Kit
AdvanCell in-cell Western Kit

Total cell normalization and simultaneous detection of two biomarkers in whole cells

  • Save time – cells are directly probed, no need for time consuming lysate preparation
  • Quantitative immunofluorescence – normalize to cell number, allowing for accurate quantification and comparison of protein expression between wells
  • Detection of high and low molecular weight targets – a limitation of traditional Western blots is eliminated as high and low MW targets can easily be evaluated simultaneously
  • Detect proteins in situ in the most relevant cellular context with minimal sample processing
AdvanStain Iris
AdvanStain Iris

High sensitivity stain for Nitrocellulose and PVDF Membranes


  • Sensitive – detect as little as 2–10ng per band
  • Rapid – detect proteins in 20 minutes or less
  • Compatible – Western blot ready, no destaining required

AdvanStain Scarlet
AdvanStain Scarlet

Fluorescent protein stain for gels and blots


  • Sensitive – detect less than a nanogram of protein per spot
  • Convenient – simple 3-hour protocol
  • Flexible – stain gels or membranes
  • Reversible – destain for downstream Western blotting or mass spectrometry
  • No speckling – clean background for better data
  • Safe – biodegradable and contains no heavy metals for increased safety and simple disposal
  • Compatible – image with any laser or CCD imaging system. Compatible excitation wavelengths include green (543, 532 nm), blue (488 nm), violet (405 nm) and UV (302, 365 nm). Maximum emission wavelength is 610 nm


Includes: AdvanStain Scarlet Dye Concentrate
Powder A, for preparation of Fix Solution
Powder B, for preparation of Stain Buffer


AdvanStain Total Fluorescent Protein Staining Kits
AdvanStain Total Fluorescent Protein Staining Kits

Rapid fluorescent stain for total protein normalization


  • Convenient – simple protocol that takes less than 20 minutes, no de-staining required
  • Versatile – compatible with chemiluminescent and IR700/IR800 fluorescent Western blotting
  • Accurate – linear across a broad range from 1-50μg of lysate, ideal for total protein normalization


Sample-loading buffer and protein stain in one

  • Instant results – see protein bands immediately as electrophoresis proceeds
  • Sensitive – detect as little as 10 to 25 ng per band
  • Fast protocol – no different from standard electrophoresis protocols - replace your current loading dye with Visio and samples labeled and ready to load after heating for 10 minutes
  • Flexible – use with reducing and non-reducing gels
  • Compatible – can be used with downstream mass spectrometry


WesternEaze-Chemi Kit
WesternEaze-Chemi Kit

Rapid, high sensitivity chemiluminescent Western blot kit for protein detection


  • Rapid – complete Western blot in 1 hour
  • Reproducible – fewer hands-on manipulations equate to consistent data
  • Convenient – contains all critical reagents required to perform a chemiluminescent Western
  • Versatile – kits are available for both mouse and rabbit primary antibodies

AdvanStain Ponceau
AdvanStain Ponceau

Protein stain for membranes


  • Quick – detect proteins in 5 minutes or less
  • Reversible – destain in minutes
  • Compatible – after destaining, PVDF and nitrocellulose membranes are ready for Western blotting