Chemiluminescent Western blotting

AdvanStain Iris
AdvanStain Iris

High sensitivity stain for Nitrocellulose and PVDF Membranes

 

  • Sensitive – detect as little as 2–10ng per band
  • Rapid – detect proteins in 20 minutes or less
  • Compatible – Western blot ready, no destaining required

AdvanStain Total Fluorescent Protein Staining Kits
AdvanStain Total Fluorescent Protein Staining Kits

Rapid fluorescent stain for total protein normalization

 

  • Convenient – simple protocol that takes less than 20 minutes, no de-staining required
  • Versatile – compatible with chemiluminescent and IR700/IR800 fluorescent Western blotting
  • Accurate – linear across a broad range from 1-50μg of lysate, ideal for total protein normalization

WesternEaze-Chemi Kit
WesternEaze-Chemi Kit

Rapid, high sensitivity chemiluminescent Western blot kit for protein detection

 

  • Rapid – complete Western blot in 1 hour
  • Reproducible – fewer hands-on manipulations equate to consistent data
  • Convenient – contains all critical reagents required to perform a chemiluminescent Western
  • Versatile – kits are available for both mouse and rabbit primary antibodies

AdvanBlock-Chemi blocking solution
AdvanBlock-Chemi blocking solution

Signal-enhancing Western blot block solution 
for the best chemiluminescent signals

  • Enhanced signal – greatly enhances Western blotting signals
  • Decreased background – decreased non-specific bands and lowered background
  • Sensitivity – get better signals for low protein sample concentrations
  • Convenience – Pre-made buffer means you save preparation and optimization time

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FLASHBlot transfer buffer
FLASHBlot transfer buffer

Ready-to-use wet transfer buffer
Designed for faster, more efficient wet protein transfer with increased sensitivity

FLASHBlot is not your average wet transfer buffer.

  • Faster than traditional wet buffers: better results in 20 minutes
  • More sensitive detection for low abundance proteins
  • Better detection of post-translationally modified proteins
  • Better transfer of ALL proteins, including high molecular weight proteins
  • Conveniently suitable for any wet transfer set-up

“ We were pleasantly surprised to see that with FLASHBlot we observed the same transferring levels at 25 min than with the lengthier transfer protocol… including for the 300 kDa protein. This reagent will shorten considerably our experimental time. ”  - Assistant Professor, UCSF

 

FLASHBlot-SD transfer buffer
FLASHBlot-SD transfer buffer

Rapid high efficiency semi-dry transfer buffer

  • Fast - high ionic strength formulation allows for protein transfer in 3 to 10 minutes when used with a compatible high current semi-dry system
  • Compatible - use your existing high current semi-dry transfer apparatus
  • Reproducible - consistent transfer across entire blot
  • Versatile - use nitrocellulose or PVDF membranes to achieve transfers with low background and high sensitivity with both chemiluminescent and fluorescent Western blots
Development folders
Development folders

Transparent plastic supports for handling and imaging blots

  • Clear images – avoid the wrinkling or bunching that can happen with plastic wrap
  • Suitable for all blots – development folders are suitable for chemiluminescence and fluorescence imaging
  • Safe blot handling – move your blot without scratching or putting pressure on the surface
  • Convenient size – image up to 2 blots simultaneously in one folder
WesternBright ECL HRP substrate
WesternBright ECL HRP substrate

The best choice for routine, film-imaged chemiluminescent Western blots

  • Stronger signal than Amersham™ ECL or Pierce™ ECL
  • Optimized – for x-ray film imaging
  • Sensitive – detect low pg protein amounts
  • Lasting signal – up to 6 hrs
  • Save money – use up to 10x less antibody

 

Includes:
WesternBright ECL Luminol/enhancer solution
WesternBright Peroxide Chemiluminescent peroxide solution

 

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WesternBright ECL Spray
WesternBright ECL Spray

The best choice for routine, film-imaged Western blots

  • Easy to use - just point and spray. No measuring or mixing required
  • Stronger signal than Amersham™ ECL or Pierce™ ECL
  • Sensitive - detect low pg protein amounts
  • Save - use up to ten-times less antibody

 

Includes:
WesternBright ECL Luminol/enhancer solution
WesternBright Peroxide Chemiluminescent peroxide solution

WesternBright Quantum HRP substrate
WesternBright Quantum HRP substrate

Quantitative chemiluminescent Western blotting

  • Sensitive – detect attomoles of protein per band.
  • Quantitative – linear range of signal with respect to protein amount exceeds 3 orders of magnitude
  • Versatile – Optimized for CCD imaging, and compatible with film detection. Chemifluorescent emissions can be detected with fluorescence imaging systems
  • Low background – for high signal to noise ratio
  • Long lasting signal – image blots hours after substrate incubation

 

Includes:
WesternBright Quantum luminol/enhancer solution
WesternBright Quantum stabilized peroxide solution

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WesternBright Sirius HRP substrate
WesternBright Sirius HRP substrate

The most sensitive chemiluminescent substrate

  • Most sensitive of Advansta's HRP substrates, for low-abundance proteins
  • Long-lasting signal for flexible imaging
  • Low background for high signal-to-noise
  • Image by CCD or film

Includes:
WesternBright™ Sirius™Luminol/enhancer solution
WesternBright™ Peroxide Chemiluminescent Detection Reagent

 

 

 

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HRP-conjugated secondary antibodies
HRP-conjugated secondary antibodies

HRP-conjugated secondary antibodies
for chemiluminescent immunodetection

  • Performance – excellent signal in immunoblotting and ELISA applications
  • Convenience – each antibody is demonstrated to provide optimal results with Advansta's protein detection systems
  • Purity – each antibody is affinity purified to maximize specificity