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Chemiluminescent Western blotting
- AdvanStain Iris
- WesternEaze-Chemi Kit
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- AdvanBlock-Chemi blocking solution
- FLASHBlot transfer buffer
- FLASHBlot-SD transfer buffer
- Development folders
- WesternBright ECL HRP substrate
- WesternBright ECL Spray
- WesternBright Quantum HRP substrate
- WesternBright Sirius HRP substrate
- HRP-conjugated secondary antibodies
- LucentBlue X-ray film
- Background Quenching Sheets
- WesternBright ChemiPen
- Transfer membranes
- Incubation trays
- AdvanWash washing solution
- Western Blot Strip-It Buffer
- Blotting sponge pads
- Blotting papers
- X-ray film cassette
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Fluorescent Western blotting
- FLASHBlot transfer buffer
- AdvanBlock-Fluor blocking solution
- AdvanBlock-PF blocking solution
- AdvanStain Total Fluorescent Protein Staining Kits
- LightSaver™ Fluorescence Enhancing Solution
- FLASHBlot-SD transfer buffer
- WesternBright MCF
- SpectraDye Secondary Antibodies
- SpectraDye Antibody Labeling Kits
- Background Quenching Sheets
- Transfer membranes
- Development folders
- Incubation trays
- AdvanWash washing solution
- Fluorescent Western standardization blot
- Blotting papers
- ELISA
- Electrophoresis
- Antibodies and antibody labeling
- Sample preparation
- Purification
- Protein staining
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Buffers and solutions
- FLASHBlot transfer buffer
- AdvanBlock-PF blocking solution
- Western Blot Strip-It Buffer
- AdvanBlock-Chemi blocking solution
- AdvanBlock-EIA blocking solution
- AdvanBlock-Fluor blocking solution
- Protein sample loading buffers
- FLASHBlot-SD transfer buffer
- AdvanWash washing solution
- 10X EIA Coating Buffer
- LightSaver™ Fluorescence Enhancing Solution
- Avant buffer pouches
- SARS-CoV-2
- Cell biology
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New special offers are available!
HRP-conjugated secondary antibodies
HRP-conjugated secondary antibodies
for chemiluminescent immunodetection
- Performance – excellent signal in immunoblotting and ELISA applications
- Convenience – each antibody is demonstrated to provide optimal results with Advansta's protein detection systems
- Purity – each antibody is affinity purified to maximize specificity
| CAT # | PRODUCT | SIZE | PRICE | QUANTITY | |
|---|---|---|---|---|---|
| R-05071-500 | Goat-anti-mouse IgG (H+L), HRP conjugate | 500ul |
$ 118.00 (USD) |
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| R-05072-500 | Goat-anti-rabbit IgG (H+L), HRP conjugate | 500ul |
$ 118.00 (USD) |
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| R-05073-500 | Goat-anti-human IgG (H+L), HRP conjugate | 500ul |
$ 118.00 (USD) |
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| R-05074-500 | Goat-anti-chicken IgY(IgG) (H+L), HRP conjugate | 500ul |
$ 227.00 (USD) |
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| R-05075-500 | Goat-anti-rat IgG (H+L), HRP conjugate | 500ul |
$ 118.00 (USD) |
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| R-05076-500 | Goat-anti-guinea pig IgG (H+L), HRP conjugate | 500ul |
$ 118.00 (USD) |
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| R-05077-500 | Donkey-anti-goat IgG (H+L), HRP conjugate | 500ul |
$ 363.00 (USD) |
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| R-05787-500 | Donkey-anti-mouse IgG (H+L), HRP conjugate | 500ul |
$ 363.00 (USD) |
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| R-05788-500 | Donkey-anti-rabbit IgG (H+L), HRP conjugate | 500ul |
$ 363.00 (USD) |
Description
Advansta’s high-quality HRP-conjugated secondary antibodies offer unparalleled sensitivity and performance for immunoblotting and ELISA applications. These secondary HRP conjugated antibodies are optimized with Advansta's protein detection systems including WesternBright ECL, WesternBright Quantum, WesternBright Sirius and ElisaBright.
Figure 1: Advansta secondary antibodies demonstrate increased sensitivity and specificity. HeLa, rat and mouse tissue lysates were separated by SDS-PAGE and transferred to a PVDF membrane. The blots were blocked with AdvanBlock™-Chemi before incubation with anti-Vinculin, anti-VDAC1 or anti-β Tubulin. Signal was detected with WesternBright® ECL. In addition to increased specificity, the blots probed with Advansta secondary antibodies demonstrate increased sensitivity compared to a leading competitor.
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