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FLASHBlot-SD transfer buffer

Rapid high efficiency semi-dry transfer buffer

Fast - high ionic strength formulation allows for protein transfer in 3 to 10 minutes when used with a compatible high current semi-dry system
Compatible - use your existing high current semi-dry transfer apparatus
Reproducible - consistent transfer across entire blot
Versatile - use nitrocellulose or PVDF membranes to achieve transfers with low background and high sensitivity with both chemiluminescent and fluorescent Western blots

CAT #	PRODUCT	SIZE	PRICE	QUANTITY
R-03104-D50	FlashBlot-SD™ Semi-Dry Transfer Buffer (1x)	500 ml	$ 137.00 (USD)		Add to cart

Description

FLASHBlot-SD Transfer Buffer is designed for rapid semi-dry transfer of proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using rapid semi-dry transfer systems. Transfer is compatible with commonly used detection methods such as membrane staining, chemiluminescent and fluorescent Western blotting.

FLASHBlot-SD produces fluorescent Western blots with low background and high sensitivity. IR fluorescent Western blot analysis of phosphorylated STAT3 and GAPDH was performed on blots containing serially diluted HeLa lysate (±IFNα treatment) that was electrophoresed by SDS-PAGE then transferred to PVDF membranes. Proteins were transferred from gel to membrane for 7 minutes at a constant current of 1.3 Amps.

FLASHBlot-SD produces chemiluminescent Western blots with highest sensitivity. Chemiluminescent Western blot analysis of hnRNP K was performed on blots containing serially diluted HeLa lysate that was electrophoresed by SDS-Page then transferred to nitrocellulose membranes. Proteins were transferred from gel to membrane for 7 minutes at a constant current of 1.3 Amps.

FLASHBlot-SD outperforms competitive semi-dry transfer buffers. (a) AdvanStain Total-PVDF fluorescent protein membrane staining was performed on blots containing serially diluted HeLa lysate that was electrophoresed by SDS-PAGE then transferred to PVDF membranes. Proteins were transferred from gel to membrane for 7 minutes at a constant current of 1.3 Amps. (b) After membrane staining was complete, an IR800 fluorescent Western blot analysis of GAPDH was performed. FLASHBlot-SD produced 4-8 fold higher Western blot sensitivity in comparison to other commercially available transfer buffers.