Buffers and solutions

AdvanBlock-Chemi blocking solution
AdvanBlock-Chemi blocking solution

Provides high sensitivity and the lowest background

  • Enhanced signal – greatly enhances Western blotting signals
  • Decreased background – decreased non-specific bands and lowered background
  • Sensitivity – get better signals for low protein sample concentrations
  • Convenience – Pre-made buffer means you save preparation and optimization time

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AdvanBlock-EIA blocking solution
AdvanBlock-EIA blocking solution

Blocking solution for ELISAs and immunoassay plates

  • Decreases background
  • Decreases well-to-well variability
  • Decreases non-specific binding
  • Ready-to-use solution
AdvanBlock-Fluor blocking solution
AdvanBlock-Fluor blocking solution

Optimized block solution 
for the best fluorescent Western blot signals

  • Enhanced signal – enhances Western blotting signals
  • Decreased background – decreased non-specific bands and lowered background
  • Sensitivity – get better signals for low protein sample concentrations
  • Convenience – Pre-made buffer means you save preparation and optimization time

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AdvanBlock-PF blocking solution
AdvanBlock-PF blocking solution

Non-protein blocking solution for fluorescent and chemiluminescent Western blotting

  • Low background - Protein-free block buffer for lower background with certain antibodies
  • Optimized - Stabilizes fluorescence of WesternBright MCF fluorescent secondary antibodies
  • Versatile - Compatible with fluorescent and chemiluminescent detection
Cleanera Endotoxin Removal Buffer Kit
Cleanera Endotoxin Removal Buffer Kit

Buffer system for cleaning various chromatography purification columns

  • Works on various types of columns: Ni-NTA, CIEX, AIEX and SEC, Protein A/G/L
  • Gentle buffer formulations that are compatible with all column matrices
  • Four sequential steps to regenerate column performance

Package Contents

  • Buffer A (50 mL)
  • Buffer B (50 mL)
  • Buffer C (50 mL)
  • Buffer D (50 mL)

Endotoxin-free Water

  • Endotoxin-free water is available separately for your convenience
FLASHBlot-SD transfer buffer
FLASHBlot-SD transfer buffer

Rapid high efficiency semi-dry transfer buffer

  • Fast - high ionic strength formulation allows for protein transfer in 3 to 10 minutes when used with a compatible high current semi-dry system
  • Compatible - use your existing high current semi-dry transfer apparatus
  • Reproducible - consistent transfer across entire blot
  • Versatile - use nitrocellulose or PVDF membranes to achieve transfers with low background and high sensitivity with both chemiluminescent and fluorescent Western blots
Protein sample loading buffers
Protein sample loading buffers

Pre-mixed standard Laemmli loading buffers for protein electrophoresis

  • Pre-mixed - just add an equal volume to your protein sample (supplied as 2x)
  • Obtain reproducible results with standardized buffers
  • Choose from reducing or non-reducing loading buffers
Western Blot Strip-It Buffer
Western Blot Strip-It Buffer

Stripping buffer for stripping antibodies from chemiluminescent Western blots

  • Conserve valuable samples - probe the same blot multiple times
  • Fast protocol - blot stripped and ready for re-probing in 25 minutes
  • Gentle protocol - antibodies removed without harsh reagents or elevated temperatures
  • Save time - no need to re-run gels and perform duplicate blots
  • Versatile - compatible with both nitrocellulose and PVDF membranes and with a variety of chemiluminescent detection reagents

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AdvanWash washing solution
AdvanWash washing solution

Washing solutions optimized for use with WesternBright systems

  • Reliable – quality controlled wash buffer for reproducible results
  • Versatile – compatible with chemiluminescent and fluorescent Western blots
  • Convenient – save time using pre-made buffers; no more weighing, mixing, or adjusting pH

 

10X EIA Coating Buffer
10X EIA Coating Buffer

Immunoassay plate coating solution

  • Reliable – quality-controlled coating buffer for reproducible results
  • Versatile – compatible with chemiluminescent and colorimetric EIA/ELISA assays
  • Convenient – save time using pre-made buffer. No more weighing, mixing or pH adjusting
LightSaver™ Fluorescence Enhancing Solution
LightSaver™ Fluorescence Enhancing Solution

Enhance sensitivity of your fluorescent Western blots

  • Improves sensitivity– Easily visualize faint bands
  • Compatible – Use with PVDF or Nitrocellulose membranes
  • Convenient – Ready-to-use solution
  • Versatile – Enhance signal of both visible and near IR fluorescent Western blots
Avant buffer pouches
Avant buffer pouches

Convenient, pure, fast buffer preparation

 

  • Quality – molecular biology-grade buffers of highest purity
  • Convenient – pre-measured powder in sealed pouches
  • Fast – dissolve and use right away
  • Standardized – guaranteed reproducible results for your electrophoresis gels and Western blots