- per page
Optimized block solution
for the best fluorescent Western blot signals
- Enhanced signal – enhances Western blotting signals
- Decreased background – decreased non-specific bands and lowered background
- Sensitivity – get better signals for low protein sample concentrations
- Convenience – Pre-made buffer means you save preparation and optimization time
Non-protein blocking solution for fluorescent and chemiluminescent Western blotting
- Low background - Protein-free block buffer for lower background with certain antibodies
- Optimized - Stabilizes fluorescence of WesternBright MCF fluorescent secondary antibodies
- Versatile - Compatible with fluorescent and chemiluminescent detection
Upgrade your protein staining! Boost sensitivity,
no-destaining, and high-contrast.
- Detection limit – 2 ng vs Ponceau at 150 ng
- Quantitative – effective for lower protein concentrations
- No destaining – allows direct chemiluminescent or fluorescent Western blotting without the need for destaining
- Versatile – compatible with both nitrocellulose and PVDF membranes
Rapid fluorescent stain for total protein normalization
- Convenient – simple protocol that takes less than 20 minutes, no de-staining required
- Versatile – compatible with chemiluminescent and IR700/IR800 fluorescent Western blotting
- Accurate – linear across a broad range from 1-50μg of lysate, ideal for total protein normalization
Rapid high efficiency semi-dry transfer buffer
- Fast - high ionic strength formulation allows for protein transfer in 3 to 10 minutes when used with a compatible high current semi-dry system
- Compatible - use your existing high current semi-dry transfer apparatus
- Reproducible - consistent transfer across entire blot
- Versatile - use nitrocellulose or PVDF membranes to achieve transfers with low background and high sensitivity with both chemiluminescent and fluorescent Western blots
Enhance sensitivity of your fluorescent Western blots
- Improves sensitivity– Easily visualize faint bands
- Compatible – Use with PVDF or Nitrocellulose membranes
- Convenient – Ready-to-use solution
- Versatile – Enhance signal of both visible and near IR fluorescent Western blots
Multi-color fluorescent Western blotting in one kit
Try multicolor fluorescent western blotting today. Our kit includes everything except primary antibodies.
- Multiplexing – visualize two proteins simultaneously!
- Sensitivity –10x greater sensitivity than Cy dyes; pictogram detection
- Quality Reagents – includes AdvanBlock™ blocking and AdvanWash-IR for washing
- Fast results – 3.5 hours for entire protocol
- Fluorescent Detection – compatible with near-IR imaging systems
Kit Includes:
Secondary antibodies
Blocking solution
Washing solution
Pre-cut low-autofluorescence PVDF membranes
Background quenching sheets
Fluorescently labeled secondary antibodies
- Fluorescent detection –secondary antibodies with a range of fluorescent conjugates
- Performance – strong signal and no cross-reactivity
- Convenience – each antibody demonstrated to provide optimal results with Advansta's Western blotting systems
- Flexibility – choose from visible or near IR fluorescent dyes and target species best suited to your application (see product listings)
Make your own fluorescent antibody in one easy step.
- Quick and easy – one-step labeling in 30 minutes
- Versatile - Labeled antibodies are compatible with immunofluorescence microscopy, flow cytometry, Western blotting
- Flexible - choose from 6 commonly used fluorescent dyes
- Save time and money – no secondary or extra steps, once you label your primary
- Save antibody – labeling reaction requires as little as 10 µg antibody
Kit includes:
Antibody Labeling Buffer
SpectraDye Dye Solution*
Quenching Solution
Neutralization Buffer
Sufficient reagents to label up to 1 mg of antibody
*Except kit K-11060-010
An added layer to decrease background
For improved imaging of blots and gels
- Better images by removing background fluorescence and stray light
- Improved signal to noise ratios
- Simple – just place sheet under the blot or gel
- Compatible with chemiluminescent and fluorescent Western blots
- Compatible with DNA gels and protein stains - including SYBR green, SYBR Gold, ethidium bromide, SYPRO® Orange, SYPRO Ruby and more
Convenient nitrocellulose and PVDF membranes for Western blotting
- Performance – quality controlled for background-free Western blots
- Convenience – membranes available pre-cut and as rolls
- High signal-to-noise ratio – low background signal for clearer results
- Flexibility – choose the right membrane for your Western blotting application
Membrane Options:
WesternBright PVDF-FL: fluorescent detection
WesternBright PVDF-CL: chemiluminescent detection; best stability for stripping and re-probing chemiluminescent blots
PVDF membranes available as rolls or pre-cut (7x9 cm, 10x15 cm, or 13x18 cm)
WesternBright NC: all-purpose nitrocellulose membrane
Nitrocellulose membranes available as rolls or pre-cut (8x10 cm)
Designed specifically for blot handling and imaging, these clear plastic folders eliminate the frustrations of traditional wrap while improving consistency and clarity
- Wrinkle-free, dust-free imaging – Avoid the wrinkling and dust that cause artifacts in imaging
- Safe handling – No pressure damage or scratching of the membrane
- Suitable for all blots – Image chemiluminescent and fluorescent blots
- Convenient storage – Easily image and store dust-free, up to two blots in each clear folder
- per page
Connect