Write or draw on your transfer membranes with the WesternBright® ChemiPen. The reagent in the ChemiPen adsorbs to nitrocellulose and PVDF membranes, and reacts with HRP substrates to produce chemiluminescence that can be detected with X-ray film or CCD imaging.
With the proprietary "ink" you can transform your visible protein markers into chemiluminescent markers, to annotate your blot with a date or blot ID, or to check the stability of your HRP substrate (Figure 1).
![ChemiPen Figure 1](/images/Figures/ChemiPen/ChemiPen_xrayfilm_comparison.jpg)
Figure 1. The WesternBright ChemiPen can be used to mark the location of protein standards and to annotate the blot with a date, as in the right panel.
The WesternBright ChemiPen has two tips, a fine tip at one end that is perfect for writing on the blot or tracing the bands of a visible protein standard, and a thicker wedge tip that can be used to deposit greater amounts of reagent on the blot. Marking the location of visible protein standards on a blot is easy. Simply trace the visible markers on the membrane after transfer and before blocking (Figure 2).
![ChemiPen Figure 2](/images/Figures/ChemiPen/ChemiPen_steps.jpg)
Figure 2. Use the WesternBright ChemiPen to visualize visible protein standards on chemiluminescent Western blots
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