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Convenient, pure, fast buffer preparation
- Quality – molecular biology-grade buffers of highest purity
- Convenient – pre-measured powder in sealed pouches
- Fast – dissolve and use right away
- Standardized – guaranteed reproducible results for your electrophoresis gels and Western blots
Immunoassay plate coating solution
- Reliable – quality-controlled coating buffer for reproducible results
- Versatile – compatible with chemiluminescent and colorimetric EIA/ELISA assays
- Convenient – save time using pre-made buffer. No more weighing, mixing or pH adjusting
LightSaver™ Fluorescence Enhancing Solution
- Enhances – fluorescent signal
- Compatible – with PVDF and Nitrocellulose membranes
- Convenient – ready-to-use solution
- Compatible – with visible and near IR fluorescent westerns
Washing solutions optimized for use with WesternBright systems
- Reliable – quality controlled wash buffer for reproducible results
- Versatile – compatible with chemiluminescent and fluorescent Western blots
- Convenient – save time using pre-made buffers; no more weighing, mixing, or adjusting pH
Signal-enhancing Western blot block solution
for the best chemiluminescent signals
- Enhanced signal – greatly enhances Western blotting signals
- Decreased background – decreased non-specific bands and lowered background
- Sensitivity – get better signals for low protein sample concentrations
- Convenience – Pre-made buffer means you save preparation and optimization time
Blocking solution for ELISAs and immunoassay plates
- Decreases background
- Decreases well-to-well variability
- Decreases non-specific binding
- Ready-to-use solution
Optimized block solution
for the best fluorescent Western blot signals
- Enhanced signal – enhances Western blotting signals
- Decreased background – decreased non-specific bands and lowered background
- Sensitivity – get better signals for low protein sample concentrations
- Convenience – Pre-made buffer means you save preparation and optimization time
Non-protein blocking solution for fluorescent and chemiluminescent Western blotting
- Low background - Protein-free block buffer for lower background with certain antibodies
- Optimized - Stabilizes fluorescence of WesternBright MCF fluorescent secondary antibodies
- Versatile - Compatible with fluorescent and chemiluminescent detection
Buffer system for cleaning various chromatography purification columns
- Works on various types of columns: Ni-NTA, CIEX, AIEX and SEC, Protein A/G/L
- Gentle buffer formulations that are compatible with all column matrices
- Four sequential steps to regenerate column performance
Package Contents
- Buffer A (50 mL)
- Buffer B (50 mL)
- Buffer C (50 mL)
- Buffer D (50 mL)
Endotoxin-free Water
- Endotoxin-free water is available separately for your convenience
Ready-to-use wet transfer buffer
Designed for faster, more efficient wet protein transfer with increased sensitivity
FLASHBlot is not your average wet transfer buffer.
- Faster than traditional wet buffers: better results in 20 minutes
- More sensitive detection for low abundance proteins
- Better detection of post-translationally modified proteins
- Better transfer of ALL proteins, including high molecular weight proteins
- Conveniently suitable for any wet transfer set-up
“ We were pleasantly surprised to see that with FLASHBlot we observed the same transferring levels at 25 min than with the lengthier transfer protocol… including for the 300 kDa protein. This reagent will shorten considerably our experimental time. ” - Assistant Professor, UCSF
Rapid high efficiency semi-dry transfer buffer
- Fast - high ionic strength formulation allows for protein transfer in 3 to 10 minutes when used with a compatible high current semi-dry system
- Compatible - use your existing high current semi-dry transfer apparatus
- Reproducible - consistent transfer across entire blot
- Versatile - use nitrocellulose or PVDF membranes to achieve transfers with low background and high sensitivity with both chemiluminescent and fluorescent Western blots
Pre-mixed standard Laemmli loading buffers for protein electrophoresis
- Pre-mixed - just add an equal volume to your protein sample (supplied as 2x)
- Obtain reproducible results with standardized buffers
- Choose from reducing or non-reducing loading buffers
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