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High sensitivity X-ray film
for chemiluminescent and other blots
- Performance - excellent images when used with WesternBright ECL
- Value - sensitivity equal to more expensive films
- Flexibility - detect radioisotopes in addition to chemiluminescence
An added layer to decrease background
For improved imaging of blots and gels
- Better images by removing background fluorescence and stray light
- Improved signal to noise ratios
- Simple – just place sheet under the blot or gel
- Compatible with chemiluminescent and fluorescent Western blots
- Compatible with DNA gels and protein stains - including SYBR green, SYBR Gold, ethidium bromide, SYPRO® Orange, SYPRO Ruby and more
A chemiluminescent marker for writing on membranes
- Improve accuracy - transform your visible protein ladder standard into a chemiluminescent standard on your Western blot
- Annotate - permanently mark your blots with the date or blot ID
- Compatible - with both X-ray film and CCD imagers
- Confirm HRP substrate stability - the ChemiPen reagent glows when incubated with an HRP substrate, so it can be used to test substrate stability
Catalog number L-15025-001 is a direct replacement for R-07055-001, which was discontinued.
Convenient nitrocellulose and PVDF membranes for Western blotting
- Performance – quality controlled for background-free Western blots
- Convenience – membranes available pre-cut and as rolls
- High signal-to-noise ratio – low background signal for clearer results
- Flexibility – choose the right membrane for your Western blotting application
Membrane Options:
WesternBright PVDF-FL: fluorescent detection
WesternBright PVDF-CL: chemiluminescent detection; best stability for stripping and re-probing chemiluminescent blots
PVDF membranes available as rolls or pre-cut (7x9 cm, 10x15 cm, or 13x18 cm)
WesternBright NC: all-purpose nitrocellulose membrane
Nitrocellulose membranes available as rolls or pre-cut (8x10 cm)
Convenient containers for staining and washing gels and membranes
- Smooth interior – protects membranes and gels from scratches
- Convenient – multiple sizes (5x7, 6x9, 9x11,10.5x15.5 cm) and designs available for different gels and blots
- Attached lid – to protect experiments from dust or debris that can cause speckles on blot images
- Save – minimize antibody and buffer usage by using appropriately sized containers
- Multi-chamber trays – process 6 mini-blots simultaneously, each chamber requires as little as 3 mL of solution
Washing solutions optimized for use with WesternBright systems
- Reliable – quality controlled wash buffer for reproducible results
- Versatile – compatible with chemiluminescent and fluorescent Western blots
- Convenient – save time using pre-made buffers; no more weighing, mixing, or adjusting pH
Stripping buffer for stripping antibodies from chemiluminescent Western blots
- Conserve valuable samples - probe the same blot multiple times
- Fast protocol - blot stripped and ready for re-probing in 25 minutes
- Gentle protocol - antibodies removed without harsh reagents or elevated temperatures
- Save time - no need to re-run gels and perform duplicate blots
- Versatile - compatible with both nitrocellulose and PVDF membranes and with a variety of chemiluminescent detection reagents
Transfer sponge pads for more reproducible Western blot transfers
- Resist compression - maintain proper pressure in transfer sandwiches without adding extra blotting papers
- Retain thickness - can be reused over multiple experiments
- Convenient - two sizes (8 x 11 cm and 9 x 15 cm) compatible with a variety of transfer apparatuses
- Standardized - uniform flat construction ensures reproducible and consistent transfers
- Four pads per pack - a full set for common transfer apparatus
Pre-cut paper for gel to membrane electrophoretic transfers
- Convenient – pre-cut to save time; sized to work with most minigel transfer assemblies
- Standardized – uniform thickness for reproducible transfer assembly
- Tested – confirmed to perform consistently in protein transfers without introducing artifacts or background noise
Light-tight film cassette for exposing blots
- Convenient - quickly load and unload with simple push-button latch
- Light weight - quality light-weight aluminum construction
- Proven performance - no light leaks even with overnight exposures
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