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The best choice for routine, film-imaged Western blots
- Easy to use - just point and spray. No measuring or mixing required
- Stronger signal than Amersham™ ECL or Pierce™ ECL
- Sensitive - detect low pg protein amounts
- Save - use up to ten-times less antibody
Includes:
WesternBright ECL Luminol/enhancer solution
WesternBright Peroxide Chemiluminescent peroxide solution
Light-tight film cassette for exposing blots
- Convenient - quickly load and unload with simple push-button latch
- Light weight - quality light-weight aluminum construction
- Proven performance - no light leaks even with overnight exposures
Rapid, high sensitivity chemiluminescent Western blot kit for protein detection
- Rapid – complete Western blot in 1 hour
- Reproducible – fewer hands-on manipulations equate to consistent data
- Convenient – contains all critical reagents required to perform a chemiluminescent Western
- Versatile – kits are available for both mouse and rabbit primary antibodies
Rapid fluorescent stain for total protein normalization
- Convenient – simple protocol that takes less than 20 minutes, no de-staining required
- Versatile – compatible with chemiluminescent and IR700/IR800 fluorescent Western blotting
- Accurate – linear across a broad range from 1-50μg of lysate, ideal for total protein normalization
Washing solutions optimized for use with WesternBright systems
- Reliable – quality controlled wash buffer for reproducible results
- Versatile – compatible with chemiluminescent and fluorescent Western blots
- Convenient – save time using pre-made buffers; no more weighing, mixing, or adjusting pH
Signal-enhancing Western blot block solution
for the best chemiluminescent signals
- Enhanced signal – greatly enhances Western blotting signals
- Decreased background – decreased non-specific bands and lowered background
- Sensitivity – get better signals for low protein sample concentrations
- Convenience – Pre-made buffer means you save preparation and optimization time
High sensitivity stain for Nitrocellulose and PVDF Membranes
- Sensitive – detect as little as 2–10ng per band
- Rapid – detect proteins in 20 minutes or less
- Compatible – Western blot ready, no destaining required
High sensitivity X-ray film
for chemiluminescent and other blots
- Performance - excellent images when used with WesternBright ECL
- Value - sensitivity equal to more expensive films
- Flexibility - detect radioisotopes in addition to chemiluminescence
Stripping buffer for stripping antibodies from chemiluminescent Western blots
- Conserve valuable samples - probe the same blot multiple times
- Fast protocol - blot stripped and ready for re-probing in 25 minutes
- Gentle protocol - antibodies removed without harsh reagents or elevated temperatures
- Save time - no need to re-run gels and perform duplicate blots
- Versatile - compatible with both nitrocellulose and PVDF membranes and with a variety of chemiluminescent detection reagents
Rapid high efficiency semi-dry transfer buffer
- Fast - high ionic strength formulation allows for protein transfer in 3 to 10 minutes when used with a compatible high current semi-dry system
- Compatible - use your existing high current semi-dry transfer apparatus
- Reproducible - consistent transfer across entire blot
- Versatile - use nitrocellulose or PVDF membranes to achieve transfers with low background and high sensitivity with both chemiluminescent and fluorescent Western blots
HRP-conjugated secondary antibodies
for chemiluminescent immunodetection
- Performance – excellent signal in immunoblotting and ELISA applications
- Convenience – each antibody is demonstrated to provide optimal results with Advansta's protein detection systems
- Purity – each antibody is affinity purified to maximize specificity
Ready-to-use wet transfer buffer
Designed for faster, more efficient wet protein transfer with increased sensitivity
FLASHBlot is not your average wet transfer buffer.
- Faster than traditional wet buffers: better results in 20 minutes
- More sensitive detection for low abundance proteins
- Better detection of post-translationally modified proteins
- Better transfer of ALL proteins, including high molecular weight proteins
- Conveniently suitable for any wet transfer set-up
“ We were pleasantly surprised to see that with FLASHBlot we observed the same transferring levels at 25 min than with the lengthier transfer protocol… including for the 300 kDa protein. This reagent will shorten considerably our experimental time. ” - Assistant Professor, UCSF
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