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Rapid, high sensitivity chemiluminescent Western blot kit for protein detection
- Rapid – complete Western blot in 1 hour
- Reproducible – fewer hands-on manipulations equate to consistent data
- Convenient – contains all critical reagents required to perform a chemiluminescent Western
- Versatile – kits are available for both mouse and rabbit primary antibodies
Rapid high efficiency semi-dry transfer buffer
- Fast - high ionic strength formulation allows for protein transfer in 3 to 10 minutes when used with a compatible high current semi-dry system
- Compatible - use your existing high current semi-dry transfer apparatus
- Reproducible - consistent transfer across entire blot
- Versatile - use nitrocellulose or PVDF membranes to achieve transfers with low background and high sensitivity with both chemiluminescent and fluorescent Western blots
Washing solutions optimized for use with WesternBright systems
- Reliable – quality controlled wash buffer for reproducible results
- Versatile – compatible with chemiluminescent and fluorescent Western blots
- Convenient – save time using pre-made buffers; no more weighing, mixing, or adjusting pH
Rapid fluorescent stain for total protein normalization
- Convenient – simple protocol that takes less than 20 minutes, no de-staining required
- Versatile – compatible with chemiluminescent and IR700/IR800 fluorescent Western blotting
- Accurate – linear across a broad range from 1-50μg of lysate, ideal for total protein normalization
Light-tight film cassette for exposing blots
- Convenient - quickly load and unload with simple push-button latch
- Light weight - quality light-weight aluminum construction
- Proven performance - no light leaks even with overnight exposures
An added layer to decrease background
For improved imaging of blots and gels
- Better images by removing background fluorescence and stray light
- Improved signal to noise ratios
- Simple – just place sheet under the blot or gel
- Compatible with chemiluminescent and fluorescent Western blots
- Compatible with DNA gels and protein stains - including SYBR green, SYBR Gold, ethidium bromide, SYPRO® Orange, SYPRO Ruby and more
Pre-cut paper for gel to membrane electrophoretic transfers
- Convenient – pre-cut to save time; sized to work with most minigel transfer assemblies
- Standardized – uniform thickness for reproducible transfer assembly
- Tested – confirmed to perform consistently in protein transfers without introducing artifacts or background noise
Ready-to-use wet transfer buffer
Designed for faster, more efficient wet protein transfer with increased sensitivity
FLASHBlot is not your average wet transfer buffer.
- Faster than traditional wet buffers: better results in 20 minutes
- More sensitive detection for low abundance proteins
- Better detection of post-translationally modified proteins
- Better transfer of ALL proteins, including high molecular weight proteins
- Conveniently suitable for any wet transfer set-up
“ We were pleasantly surprised to see that with FLASHBlot we observed the same transferring levels at 25 min than with the lengthier transfer protocol… including for the 300 kDa protein. This reagent will shorten considerably our experimental time. ” - Assistant Professor, UCSF
Elevate your Western blot results with our chemiluminescent substrate, backed by over 2000 citations for its exceptional sensitivity and up to 10X less antibody usage. Our user-friendly spray device simplifies your workflow – no pipetting, measuring, or mixing needed.
- Convenience – Just point and spray. No pipetting, measuring, or mixing required
- High sensitivity – Detect low pg protein amount with film or digital imaging
- Reduce waste – Eliminate use of tips and tubes for substrate mixing
- Easy-to-use – Both components of ECL substrate in one convenient spray device
Includes:
WesternBright ECL Luminol/enhancer solution
WesternBright Peroxide Chemiluminescent peroxide solution
Upgrade your protein staining! Boost sensitivity,
no-destaining, and high-contrast.
- Detection limit – 2 ng vs Ponceau at 150 ng
- Quantitative – effective for lower protein concentrations
- No destaining – allows direct chemiluminescent or fluorescent Western blotting without the need for destaining
- Versatile – compatible with both nitrocellulose and PVDF membranes
HRP-conjugated secondary antibodies
for chemiluminescent immunodetection
- Performance – excellent signal in immunoblotting and ELISA applications
- Convenience – each antibody is demonstrated to provide optimal results with Advansta's protein detection systems
- Purity – each antibody is affinity purified to maximize specificity
A chemiluminescent marker for writing on membranes
- Improve accuracy - transform your visible protein ladder standard into a chemiluminescent standard on your Western blot
- Annotate - permanently mark your blots with the date or blot ID
- Compatible - with both X-ray film and CCD imagers
- Confirm HRP substrate stability - the ChemiPen reagent glows when incubated with an HRP substrate, so it can be used to test substrate stability
Catalog number L-15025-001 is a direct replacement for R-07055-001, which was discontinued.
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