Chemiluminescent Western blotting

WesternEaze-Chemi Kit
WesternEaze-Chemi Kit

Rapid, high sensitivity chemiluminescent Western blot kit for protein detection

 

  • Rapid – complete Western blot in 1 hour
  • Reproducible – fewer hands-on manipulations equate to consistent data
  • Convenient – contains all critical reagents required to perform a chemiluminescent Western
  • Versatile – kits are available for both mouse and rabbit primary antibodies

FLASHBlot-SD transfer buffer
FLASHBlot-SD transfer buffer

Rapid high efficiency semi-dry transfer buffer

  • Fast - high ionic strength formulation allows for protein transfer in 3 to 10 minutes when used with a compatible high current semi-dry system
  • Compatible - use your existing high current semi-dry transfer apparatus
  • Reproducible - consistent transfer across entire blot
  • Versatile - use nitrocellulose or PVDF membranes to achieve transfers with low background and high sensitivity with both chemiluminescent and fluorescent Western blots
AdvanWash washing solution
AdvanWash washing solution

Washing solutions optimized for use with WesternBright systems

  • Reliable – quality controlled wash buffer for reproducible results
  • Versatile – compatible with chemiluminescent and fluorescent Western blots
  • Convenient – save time using pre-made buffers; no more weighing, mixing, or adjusting pH

 

AdvanStain Total Fluorescent Protein Staining Kits
AdvanStain Total Fluorescent Protein Staining Kits

Rapid fluorescent stain for total protein normalization

 

  • Convenient – simple protocol that takes less than 20 minutes, no de-staining required
  • Versatile – compatible with chemiluminescent and IR700/IR800 fluorescent Western blotting
  • Accurate – linear across a broad range from 1-50μg of lysate, ideal for total protein normalization

Background Quenching Sheets
Background Quenching Sheets

An added layer to decrease background
For improved imaging of blots and gels

  • Better images by removing background fluorescence and stray light
  • Improved signal to noise ratios
  • Simple – just place sheet under the blot or gel
  • Compatible with chemiluminescent and fluorescent Western blots
  • Compatible with DNA gels and protein stains - including SYBR green, SYBR Gold, ethidium bromide, SYPRO® Orange, SYPRO Ruby and more

 

X-ray film cassette
X-ray film cassette

Light-tight film cassette for exposing blots

  •  Convenient - quickly load and unload with simple push-button latch
  • Light weight - quality light-weight aluminum construction
  • Proven performance - no light leaks even with overnight exposures
Blotting papers
Blotting papers

Pre-cut paper for electrophoretic transfers

  • Convenient – pre-cut; work with most mini gel transfer assemblies
  • Standardized – uniform thickness for reproducible transfer
  • Tested – perform consistently without artifacts or background
  • Available sizes include 7x9 cm and 8x10 cm
AdvanStain Iris
AdvanStain Iris

High sensitivity stain for Nitrocellulose and PVDF Membranes

 

  • Sensitive – detect as little as 2–10ng per band
  • Rapid – detect proteins in 20 minutes or less
  • Compatible – Western blot ready, no destaining required

FLASHBlot transfer buffer
FLASHBlot transfer buffer

Ready-to-use wet transfer buffer
Designed for faster, more efficient wet protein transfer with increased sensitivity

FLASHBlot is not your average wet transfer buffer.

  • Faster than traditional wet buffers: better results in 20 minutes
  • More sensitive detection for low abundance proteins
  • Better detection of post-translationally modified proteins
  • Better transfer of ALL proteins, including high molecular weight proteins
  • Conveniently suitable for any wet transfer set-up

“ We were pleasantly surprised to see that with FLASHBlot we observed the same transferring levels at 25 min than with the lengthier transfer protocol… including for the 300 kDa protein. This reagent will shorten considerably our experimental time. ”  - Assistant Professor, UCSF

 

WesternBright ECL Spray
WesternBright ECL Spray

The best choice for routine, film-imaged Western blots

  • Easy to use - just point and spray. No measuring or mixing required
  • Stronger signal than Amersham™ ECL or Pierce™ ECL
  • Sensitive - detect low pg protein amounts
  • Save - use up to ten-times less antibody

 

Includes:
WesternBright ECL Luminol/enhancer solution
WesternBright Peroxide Chemiluminescent peroxide solution

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HRP-conjugated secondary antibodies
HRP-conjugated secondary antibodies

HRP-conjugated secondary antibodies
for chemiluminescent immunodetection

  • Performance – excellent signal in immunoblotting and ELISA applications
  • Convenience – each antibody is demonstrated to provide optimal results with Advansta's protein detection systems
  • Purity – each antibody is affinity purified to maximize specificity
WesternBright ChemiPen
WesternBright ChemiPen

A chemiluminescent marker for writing on membranes

  • Improve accuracy - transform your visible protein ladder standard into a chemiluminescent standard on your Western blot
  • Annotate - permanently mark your blots with the date or blot ID
  • Compatible - with both X-ray film and CCD imagers
  • Confirm HRP substrate stability - the ChemiPen reagent glows when incubated with an HRP substrate, so it can be used to test substrate stability