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Light-tight film cassette for exposing blots
- Convenient - quickly load and unload with simple push-button latch
- Light weight - quality light-weight aluminum construction
- Proven performance - no light leaks even with overnight exposures
Rapid, high sensitivity chemiluminescent Western blot kit for protein detection
- Rapid – complete Western blot in 1 hour
- Reproducible – fewer hands-on manipulations equate to consistent data
- Convenient – contains all critical reagents required to perform a chemiluminescent Western
- Versatile – kits are available for both mouse and rabbit primary antibodies
The most sensitive chemiluminescent substrate
- Most sensitive of Advansta's HRP substrates, for low-abundance proteins
- Long-lasting signal for flexible imaging
- Low background for high signal-to-noise
- Image by CCD or film
Includes:
WesternBright™ Sirius™Luminol/enhancer solution
WesternBright™ Peroxide Chemiluminescent Detection Reagent
Westernbright Quantum was chemically engineered for digital detection to maximize the data from each blot
- High sensitivity – Detect very low amounts of protein down to femtogram levels
- Ultra-low background – Faint bands are clearly seen without high general background
- Long-lasting signal – Capture multiple exposures without worrying about signal decay
- Linear signal over broad dynamic range – Low and high abundance proteins can be imaged simultaneously with a range spanning over three orders of magnitude
Includes:
WesternBright Quantum luminol/enhancer solution
WesternBright Quantum stabilized peroxide solution
Elevate your Western blot results with our chemiluminescent substrate, backed by over 2000 citations for its exceptional sensitivity and up to 10X less antibody usage. Our user-friendly spray device simplifies your workflow – no pipetting, measuring, or mixing needed.
- Convenience – Just point and spray. No pipetting, measuring, or mixing required
- High sensitivity – Detect low pg protein amount with film or digital imaging
- Reduce waste – Eliminate use of tips and tubes for substrate mixing
- Easy-to-use – Both components of ECL substrate in one convenient spray device
Includes:
WesternBright ECL Luminol/enhancer solution
WesternBright Peroxide Chemiluminescent peroxide solution
Advanced HRP substrate for brighter Westerns
- Optimized – For shorter exposure times
- High sensitivity – Detect low pg protein amount with film or digital imaging
- Long-lasting signal – Capture multiple exposures without worrying about signal decay
- Save money – Use up to 10X less antibody
Includes:
WesternBright ECL Luminol/enhancer solution
WesternBright Peroxide Chemiluminescent peroxide solution
A chemiluminescent marker for writing on membranes
- Improve accuracy - transform your visible protein ladder standard into a chemiluminescent standard on your Western blot
- Annotate - permanently mark your blots with the date or blot ID
- Compatible - with both X-ray film and CCD imagers
- Confirm HRP substrate stability - the ChemiPen reagent glows when incubated with an HRP substrate, so it can be used to test substrate stability
Catalog number L-15025-001 is a direct replacement for R-07055-001, which was discontinued.
Stripping buffer for stripping antibodies from chemiluminescent Western blots
- Conserve valuable samples - probe the same blot multiple times
- Fast protocol - blot stripped and ready for re-probing in 25 minutes
- Gentle protocol - antibodies removed without harsh reagents or elevated temperatures
- Save time - no need to re-run gels and perform duplicate blots
- Versatile - compatible with both nitrocellulose and PVDF membranes and with a variety of chemiluminescent detection reagents
Convenient nitrocellulose and PVDF membranes for Western blotting
- Performance – quality controlled for background-free Western blots
- Convenience – membranes available pre-cut and as rolls
- High signal-to-noise ratio – low background signal for clearer results
- Flexibility – choose the right membrane for your Western blotting application
Membrane Options:
WesternBright PVDF-FL: fluorescent detection
WesternBright PVDF-CL: chemiluminescent detection; best stability for stripping and re-probing chemiluminescent blots
PVDF membranes available as rolls or pre-cut (7x9 cm, 10x15 cm, or 13x18 cm)
WesternBright NC: all-purpose nitrocellulose membrane
Nitrocellulose membranes available as rolls or pre-cut (8x10 cm)
High sensitivity X-ray film
for chemiluminescent and other blots
- Performance - excellent images when used with WesternBright ECL
- Value - sensitivity equal to more expensive films
- Flexibility - detect radioisotopes in addition to chemiluminescence
Convenient containers for staining and washing gels and membranes
- Smooth interior – protects membranes and gels from scratches
- Convenient – multiple sizes (5x7, 6x9, 9x11,10.5x15.5 cm) and designs available for different gels and blots
- Attached lid – to protect experiments from dust or debris that can cause speckles on blot images
- Save – minimize antibody and buffer usage by using appropriately sized containers
- Multi-chamber trays – process 6 mini-blots simultaneously, each chamber requires as little as 3 mL of solution
HRP-conjugated secondary antibodies
for chemiluminescent immunodetection
- Performance – excellent signal in immunoblotting and ELISA applications
- Convenience – each antibody is demonstrated to provide optimal results with Advansta's protein detection systems
- Purity – each antibody is affinity purified to maximize specificity
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