Fluorescent Western blotting

AdvanBlock-Fluor blocking solution
AdvanBlock-Fluor blocking solution

Optimized block solution 
for the best fluorescent Western blot signals

  • Enhanced signal – enhances Western blotting signals
  • Decreased background – decreased non-specific bands and lowered background
  • Sensitivity – get better signals for low protein sample concentrations
  • Convenience – Pre-made buffer means you save preparation and optimization time

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AdvanStain Iris
AdvanStain Iris

Upgrade your protein staining! Boost sensitivity,
no-destaining, and high-contrast.

  • Detection limit – 2 ng vs Ponceau at 150 ng
  • Quantitative – effective for lower protein concentrations
  • No destaining – allows direct chemiluminescent or fluorescent Western blotting without the need for destaining
  • Versatile – compatible with both nitrocellulose and PVDF membranes

 

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Transfer membranes
Transfer membranes

Convenient nitrocellulose and PVDF membranes for Western blotting

  • Performance – quality controlled for background-free Western blots
  • Convenience – membranes available pre-cut and as rolls
  • High signal-to-noise ratio – low background signal for clearer results
  • Flexibility – choose the right membrane for your Western blotting application

 

Membrane Options:

WesternBright PVDF-FL: fluorescent detection
WesternBright PVDF-CL: chemiluminescent detection; best stability for stripping and re-probing chemiluminescent blots
PVDF membranes available as rolls or pre-cut (7x9 cm, 10x15 cm, or 13x18 cm)

WesternBright NC: all-purpose nitrocellulose membrane
Nitrocellulose membranes available as rolls or pre-cut (8x10 cm)

Development folders
Development folders

Designed specifically for blot handling and imaging, these clear plastic folders eliminate the frustrations of traditional wrap while improving consistency and clarity

  • Wrinkle-free, dust-free imaging – Avoid the wrinkling and dust that cause artifacts in imaging
  • Safe handling – No pressure damage or scratching of the membrane
  • Suitable for all blots – Image chemiluminescent and fluorescent blots
  • Convenient storage – Easily image and store dust-free, up to two blots in each clear folder
Incubation trays
Incubation trays

Convenient containers for staining and washing gels and membranes

  • Smooth interior – protects membranes and gels from scratches
  • Convenient – multiple sizes (5x7, 6x9, 9x11,10.5x15.5 cm) and designs available for different gels and blots
  • Attached lid – to protect experiments from dust or debris that can cause speckles on blot images
  • Save – minimize antibody and buffer usage by using appropriately sized containers
  • Multi-chamber trays – process 6 mini-blots simultaneously, each chamber requires as little as 3 mL of solution