Fluorescent Western blotting

Blotting papers
Blotting papers

Pre-cut paper for electrophoretic transfers

  • Convenient – pre-cut; work with most mini gel transfer assemblies
  • Standardized – uniform thickness for reproducible transfer
  • Tested – perform consistently without artifacts or background
  • Available sizes include 7x9 cm and 8x10 cm
Fluorescent Western standardization blot
Fluorescent Western standardization blot

Ready-to-image three-color fluorescent blot

  • Fluorescently labeled primary and secondary antibodies fluoresce in the red, green, and blue fluorescent channels (like Cy2, Cy3, and Cy5 equivalent dyes)
  • Convenient – an easy way to evaluate the performance of your fluorescence imaging system
  • Reliable – quality controlled, with lot-specific statistics provided for every blot
AdvanWash washing solution
AdvanWash washing solution

Washing solutions optimized for use with WesternBright systems

  • Reliable – quality controlled wash buffer for reproducible results
  • Versatile – compatible with chemiluminescent and fluorescent Western blots
  • Convenient – save time using pre-made buffers; no more weighing, mixing, or adjusting pH

 

Incubation trays
Incubation trays

Convenient containers for staining and washing gels and membranes

  • Smooth interior – protects membranes and gels from scratches
  • Convenient – multiple sizes (5x7, 6x9, 9x11,10.5x15.5 cm) and designs available for different gels and blots
  • Attached lid – to protect experiments from dust or debris that can cause speckles on blot images
  • Save – minimize antibody and buffer usage by using appropriately sized containers
  • Multi-chamber trays – process 6 mini-blots simultaneously, each chamber requires as little as 3 mL of solution

 

Development folders
Development folders

Transparent plastic supports for handling and imaging blots

  • Clear images – avoid the wrinkling or bunching that can happen with plastic wrap
  • Suitable for all blots – development folders are suitable for chemiluminescence and fluorescence imaging
  • Safe blot handling – move your blot without scratching or putting pressure on the surface
  • Convenient size – image up to 2 blots simultaneously in one folder
Transfer membranes
Transfer membranes

Convenient nitrocellulose and PVDF membranes for Western blotting

  • Performance – quality controlled for background-free Western blots
  • Convenience – membranes available pre-cut and as rolls
  • High signal-to-noise ratio – low background signal for clearer results
  • Flexibility – choose the right membrane for your Western blotting application

 

Membrane Options:

WesternBright PVDF-FL: fluorescent detection
WesternBright PVDF-CL: chemiluminescent detection; best stability for stripping and re-probing chemiluminescent blots
PVDF membranes available as rolls or pre-cut (7x9 cm, 10x15 cm, or 13x18 cm)

WesternBright NC: all-purpose nitrocellulose membrane
Nitrocellulose membranes available as rolls or pre-cut (8x10 cm)

Background Quenching Sheets
Background Quenching Sheets

An added layer to decrease background
For improved imaging of blots and gels

  • Better images by removing background fluorescence and stray light
  • Improved signal to noise ratios
  • Simple – just place sheet under the blot or gel
  • Compatible with chemiluminescent and fluorescent Western blots
  • Compatible with DNA gels and protein stains - including SYBR green, SYBR Gold, ethidium bromide, SYPRO® Orange, SYPRO Ruby and more

 

SpectraDye Antibody Labeling Kits
SpectraDye Antibody Labeling Kits

Make your own fluorescent antibody in one easy step.

  • Quick and easy – one-step labeling in 30 minutes
  • Versatile - Labeled antibodies are compatible with immunofluorescence microscopy, flow cytometry, Western blotting
  • Flexible - choose from 6 commonly used fluorescent dyes
  • Save time and money – no secondary or extra steps, once you label your primary
  • Save antibody – labeling reaction requires as little as 10 µg antibody

 

Kit includes:
Antibody Labeling Buffer
SpectraDye Dye Solution*
Quenching Solution
Neutralization Buffer

Sufficient reagents to label up to 1 mg of antibody
*Except kit K-11060-010

 

Spectradyes

SpectraDye Secondary Antibodies
SpectraDye Secondary Antibodies

Fluorescently labeled secondary antibodies

  • Fluorescent detection –secondary antibodies with a range of fluorescent conjugates
  • Performance – strong signal and no cross-reactivity
  • Convenience – each antibody demonstrated to provide optimal results with Advansta's Western blotting systems
  • Flexibility – choose from visible or near IR fluorescent dyes and target species best suited to your application (see product listings)

2nd AB excitation emission chart

 

WesternBright MCF
WesternBright MCF

Multi-color fluorescent Western blotting in one kit

Try multicolor fluorescent western blotting today. Our kit includes everything except primary antibodies.

  • Multiplexing – visualize two proteins simultaneously!
  • Sensitivity –10x greater sensitivity than Cy dyes; pictogram detection
  • Quality Reagents – includes AdvanBlock™-PF blocking and AdvanWash /AdvanWash-IR for washing
  • Fast results – 3.5 hours for entire protocol
  • Compatible with imaging systems that detect Cy3 and Cy5 (MCF) or near-IR imaging systems (MCF-IR)
  • Fluorescent Detection – choose between visible (APC and RPE) or near IR (IR700 and IR800) kits with SpectraDye™ secondary antibodies

 

Kit Includes:
Secondary antibodies
Blocking solution
Washing solution
Pre-cut low-autofluorescence PVDF membranes
Background quenching sheets

 

AdvanBlock-Fluor blocking solution
AdvanBlock-Fluor blocking solution

Optimized block solution 
for the best fluorescent Western blot signals

  • Enhanced signal – enhances Western blotting signals
  • Decreased background – decreased non-specific bands and lowered background
  • Sensitivity – get better signals for low protein sample concentrations
  • Convenience – Pre-made buffer means you save preparation and optimization time

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AdvanBlock-PF blocking solution
AdvanBlock-PF blocking solution

Non-protein blocking solution for fluorescent and chemiluminescent Western blotting

  • Low background - Protein-free block buffer for lower background with certain antibodies
  • Optimized - Stabilizes fluorescence of WesternBright MCF fluorescent secondary antibodies
  • Versatile - Compatible with fluorescent and chemiluminescent detection