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Pre-cut paper for electrophoretic transfers
- Convenient – pre-cut; work with most mini gel transfer assemblies
- Standardized – uniform thickness for reproducible transfer
- Tested – perform consistently without artifacts or background
- Available sizes include 7x9 cm and 8x10 cm
Ready-to-image three-color fluorescent blot
- Fluorescently labeled primary and secondary antibodies fluoresce in the red, green, and blue fluorescent channels (like Cy2, Cy3, and Cy5 equivalent dyes)
- Convenient – an easy way to evaluate the performance of your fluorescence imaging system
- Reliable – quality controlled, with lot-specific statistics provided for every blot
Washing solutions optimized for use with WesternBright systems
- Reliable – quality controlled wash buffer for reproducible results
- Versatile – compatible with chemiluminescent and fluorescent Western blots
- Convenient – save time using pre-made buffers; no more weighing, mixing, or adjusting pH
Convenient containers for staining and washing gels and membranes
- Smooth interior – protects membranes and gels from scratches
- Convenient – multiple sizes (5x7, 6x9, 9x11,10.5x15.5 cm) and designs available for different gels and blots
- Attached lid – to protect experiments from dust or debris that can cause speckles on blot images
- Save – minimize antibody and buffer usage by using appropriately sized containers
- Multi-chamber trays – process 6 mini-blots simultaneously, each chamber requires as little as 3 mL of solution
Transparent plastic supports for handling and imaging blots
- Clear images – avoid the wrinkling or bunching that can happen with plastic wrap
- Suitable for all blots – development folders are suitable for chemiluminescence and fluorescence imaging
- Safe blot handling – move your blot without scratching or putting pressure on the surface
- Convenient size – image up to 2 blots simultaneously in one folder
Convenient nitrocellulose and PVDF membranes for Western blotting
- Performance – quality controlled for background-free Western blots
- Convenience – membranes available pre-cut and as rolls
- High signal-to-noise ratio – low background signal for clearer results
- Flexibility – choose the right membrane for your Western blotting application
Membrane Options:
WesternBright PVDF-FL: fluorescent detection
WesternBright PVDF-CL: chemiluminescent detection; best stability for stripping and re-probing chemiluminescent blots
PVDF membranes available as rolls or pre-cut (7x9 cm, 10x15 cm, or 13x18 cm)
WesternBright NC: all-purpose nitrocellulose membrane
Nitrocellulose membranes available as rolls or pre-cut (8x10 cm)
An added layer to decrease background
For improved imaging of blots and gels
- Better images by removing background fluorescence and stray light
- Improved signal to noise ratios
- Simple – just place sheet under the blot or gel
- Compatible with chemiluminescent and fluorescent Western blots
- Compatible with DNA gels and protein stains - including SYBR green, SYBR Gold, ethidium bromide, SYPRO® Orange, SYPRO Ruby and more
Make your own fluorescent antibody in one easy step.
- Quick and easy – one-step labeling in 30 minutes
- Versatile - Labeled antibodies are compatible with immunofluorescence microscopy, flow cytometry, Western blotting
- Flexible - choose from 6 commonly used fluorescent dyes
- Save time and money – no secondary or extra steps, once you label your primary
- Save antibody – labeling reaction requires as little as 10 µg antibody
Kit includes:
Antibody Labeling Buffer
SpectraDye Dye Solution*
Quenching Solution
Neutralization Buffer
Sufficient reagents to label up to 1 mg of antibody
*Except kit K-11060-010
Fluorescently labeled secondary antibodies
- Fluorescent detection –secondary antibodies with a range of fluorescent conjugates
- Performance – strong signal and no cross-reactivity
- Convenience – each antibody demonstrated to provide optimal results with Advansta's Western blotting systems
- Flexibility – choose from visible or near IR fluorescent dyes and target species best suited to your application (see product listings)
Multi-color fluorescent Western blotting in one kit
Try multicolor fluorescent western blotting today. Our kit includes everything except primary antibodies.
- Multiplexing – visualize two proteins simultaneously!
- Sensitivity –10x greater sensitivity than Cy dyes; pictogram detection
- Quality Reagents – includes AdvanBlock™-PF blocking and AdvanWash /AdvanWash-IR for washing
- Fast results – 3.5 hours for entire protocol
- Compatible with imaging systems that detect Cy3 and Cy5 (MCF) or near-IR imaging systems (MCF-IR)
- Fluorescent Detection – choose between visible (APC and RPE) or near IR (IR700 and IR800) kits with SpectraDye™ secondary antibodies
Kit Includes:
Secondary antibodies
Blocking solution
Washing solution
Pre-cut low-autofluorescence PVDF membranes
Background quenching sheets
Optimized block solution
for the best fluorescent Western blot signals
- Enhanced signal – enhances Western blotting signals
- Decreased background – decreased non-specific bands and lowered background
- Sensitivity – get better signals for low protein sample concentrations
- Convenience – Pre-made buffer means you save preparation and optimization time
Non-protein blocking solution for fluorescent and chemiluminescent Western blotting
- Low background - Protein-free block buffer for lower background with certain antibodies
- Optimized - Stabilizes fluorescence of WesternBright MCF fluorescent secondary antibodies
- Versatile - Compatible with fluorescent and chemiluminescent detection
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