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Sample preparation

Protein sample loading buffers
Protein sample loading buffers

Pre-mixed standard Laemmli loading buffers for protein electrophoresis

  • Pre-mixed - just add an equal volume to your protein sample (supplied as 2x)
  • Obtain reproducible results with standardized buffers
  • Choose from reducing or non-reducing loading buffers
Afyon SDS-PAGE sample preparation kit
Afyon SDS-PAGE sample preparation kit

Kit for fast, easy concentration and purification of samples for SDS-PAGE

 

  • Purify - Remove contaminants that can interfere with electrophoresis (GuHCl, urea, Ammonium sulfate, etc)
  • Quick - Protein samples ready to load in less than 10 minutes
  • Safe - and non-toxic - no DMSO required
  • Compatible with SDS-PAGE and downstream Western blotting
  • Many times faster than concentration and buffer exchange via ultrafiltration or dialysis

 

G-25 Desalting Spin Columns
G-25 Desalting Spin Columns

Rapid buffer exchange for protein samples

 

  • Fast protocol – buffer exchange or salt removal complete in less than 10 minutes
  • Convenient – pre-made single-use columns
  • Performance – high desalting capacity
  • Versatile – may be used for multiple applications including desalting, buffer exchange, and removal of free dye or any compound with molecular weight below 5000
Cleanera Endotoxin Removal Buffer Kit
Cleanera Endotoxin Removal Buffer Kit

Buffer system for cleaning various chromatography purification columns

  • Works on various types of columns: Ni-NTA, CIEX, AIEX and SEC, Protein A/G/L
  • Gentle buffer formulations that are compatible with all column matrices
  • Four sequential steps to regenerate column performance

Package Contents

  • Buffer A (50 mL)
  • Buffer B (50 mL)
  • Buffer C (50 mL)
  • Buffer D (50 mL)

Endotoxin-free Water

  • Endotoxin-free water is available separately for your convenience