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Antibodies and antibody labeling

SpectraDye Secondary Antibodies
SpectraDye Secondary Antibodies

Fluorescently labeled secondary antibodies

  • Fluorescent detection –secondary antibodies with a range of fluorescent conjugates
  • Performance – strong signal and no cross-reactivity
  • Convenience – each antibody demonstrated to provide optimal results with Advansta's Western blotting systems
  • Flexibility – choose from visible or near IR fluorescent dyes and target species best suited to your application (see product listings)

2nd AB excitation emission chart

 

G-25 Desalting Spin Columns
G-25 Desalting Spin Columns

Rapid buffer exchange for protein samples

 

  • Fast protocol – buffer exchange or salt removal complete in less than 10 minutes
  • Convenient – pre-made single-use columns
  • Performance – high desalting capacity
  • Versatile – may be used for multiple applications including desalting, buffer exchange, and removal of free dye or any compound with molecular weight below 5000
HRP-conjugated secondary antibodies
HRP-conjugated secondary antibodies

HRP-conjugated secondary antibodies
for chemiluminescent immunodetection

  • Performance – excellent signal in immunoblotting and ELISA applications
  • Convenience – each antibody is demonstrated to provide optimal results with Advansta's protein detection systems
  • Purity – each antibody is affinity purified to maximize specificity
SpectraDye Antibody Labeling Kits
SpectraDye Antibody Labeling Kits

Make your own fluorescent antibody in one easy step.

  • Quick and easy – one-step labeling in 30 minutes
  • Versatile - Labeled antibodies are compatible with immunofluorescence microscopy, flow cytometry, Western blotting
  • Flexible - choose from 6 commonly used fluorescent dyes
  • Save time and money – no secondary or extra steps, once you label your primary
  • Save antibody – labeling reaction requires as little as 10 µg antibody

 

Kit includes:
Antibody Labeling Buffer
SpectraDye Dye Solution*
Quenching Solution
Neutralization Buffer

Sufficient reagents to label up to 1 mg of antibody
*Except kit K-11060-010