AdvanCell in-cell Western Kit
Total cell normalization and simultaneous detection of two biomarkers in whole cells
- Save time – cells are directly probed, no need for time consuming lysate preparation
- Quantitative immunofluorescence – normalize to cell number, allowing for accurate quantification and comparison of protein expression between wells
- Detection of high and low molecular weight targets – a limitation of traditional Western blots is eliminated as high and low MW targets can easily be evaluated simultaneously
- Detect proteins in situ in the most relevant cellular context with minimal sample processing
G-25 Desalting Spin Columns
Rapid buffer exchange for protein samples
- Fast protocol – buffer exchange or salt removal complete in less than 10 minutes
- Convenient – pre-made single-use columns
- Performance – high desalting capacity
- Versatile – may be used for multiple applications including desalting, buffer exchange, and removal of free dye or any compound with molecular weight below 5000
HRP-conjugated secondary antibodies
HRP-conjugated secondary antibodies
for chemiluminescent immunodetection
- Performance – excellent signal in immunoblotting and ELISA applications
- Convenience – each antibody is demonstrated to provide optimal results with Advansta's protein detection systems
- Purity – each antibody is affinity purified to maximize specificity
SpectraDye Antibody Labeling Kits
Make your own fluorescent antibody in one easy step.
- Quick and easy – one-step labeling in 30 minutes
- Versatile - Labeled antibodies are compatible with immunofluorescence microscopy, flow cytometry, Western blotting
- Flexible - choose from 6 commonly used fluorescent dyes
- Save time and money – no secondary or extra steps, once you label your primary
- Save antibody – labeling reaction requires as little as 10 µg antibody
Kit includes:
Antibody Labeling Buffer
SpectraDye Dye Solution*
Quenching Solution
Neutralization Buffer
Sufficient reagents to label up to 1 mg of antibody
*Except kit K-11060-010
SpectraDye Secondary Antibodies
Fluorescently labeled secondary antibodies
- Fluorescent detection –secondary antibodies with a range of fluorescent conjugates
- Performance – strong signal and no cross-reactivity
- Convenience – each antibody demonstrated to provide optimal results with Advansta's Western blotting systems
- Flexibility – choose from visible or near IR fluorescent dyes and target species best suited to your application (see product listings)
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