Chemiluminescent Western blotting

FLASHBlot-SD transfer buffer
FLASHBlot-SD transfer buffer

Rapid high efficiency semi-dry transfer buffer

  • Fast - high ionic strength formulation allows for protein transfer in 3 to 10 minutes when used with a compatible high current semi-dry system
  • Compatible - use your existing high current semi-dry transfer apparatus
  • Reproducible - consistent transfer across entire blot
  • Versatile - use nitrocellulose or PVDF membranes to achieve transfers with low background and high sensitivity with both chemiluminescent and fluorescent Western blots
Western Blot Strip-It Buffer
Western Blot Strip-It Buffer

Stripping buffer for stripping antibodies from chemiluminescent Western blots

  • Conserve valuable samples - probe the same blot multiple times
  • Fast protocol - blot stripped and ready for re-probing in 25 minutes
  • Gentle protocol - antibodies removed without harsh reagents or elevated temperatures
  • Save time - no need to re-run gels and perform duplicate blots
  • Versatile - compatible with both nitrocellulose and PVDF membranes and with a variety of chemiluminescent detection reagents

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LucentBlue X-ray film
LucentBlue X-ray film

High sensitivity X-ray film
for chemiluminescent and other blots

  • Performance - excellent images when used with WesternBright ECL
  • Value - sensitivity equal to more expensive films
  • Flexibility - detect radioisotopes in addition to chemiluminescence
AdvanStain Iris
AdvanStain Iris

Upgrade your protein staining! Boost sensitivity,
no-destaining, and high-contrast.

  • Detection limit – 2 ng vs Ponceau at 150 ng
  • Quantitative – effective for lower protein concentrations
  • No destaining – allows direct chemiluminescent or fluorescent Western blotting without the need for destaining
  • Versatile – compatible with both nitrocellulose and PVDF membranes

 

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AdvanBlock-Chemi blocking solution
AdvanBlock-Chemi blocking solution

Provides high sensitivity and the lowest background

  • Enhanced signal – greatly enhances Western blotting signals
  • Decreased background – decreased non-specific bands and lowered background
  • Sensitivity – get better signals for low protein sample concentrations
  • Convenience – Pre-made buffer means you save preparation and optimization time

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AdvanWash washing solution
AdvanWash washing solution

Washing solutions optimized for use with WesternBright systems

  • Reliable – quality controlled wash buffer for reproducible results
  • Versatile – compatible with chemiluminescent and fluorescent Western blots
  • Convenient – save time using pre-made buffers; no more weighing, mixing, or adjusting pH

 

AdvanStain Total Fluorescent Protein Staining Kits
AdvanStain Total Fluorescent Protein Staining Kits

Rapid fluorescent stain for total protein normalization

 

  • Convenient – simple protocol that takes less than 20 minutes, no de-staining required
  • Versatile – compatible with chemiluminescent and IR700/IR800 fluorescent Western blotting
  • Accurate – linear across a broad range from 1-50μg of lysate, ideal for total protein normalization

WesternEaze-Chemi Kit
WesternEaze-Chemi Kit

Rapid, high sensitivity chemiluminescent Western blot kit for protein detection

 

  • Rapid – complete Western blot in 1 hour
  • Reproducible – fewer hands-on manipulations equate to consistent data
  • Convenient – contains all critical reagents required to perform a chemiluminescent Western
  • Versatile – kits are available for both mouse and rabbit primary antibodies

X-ray film cassette
X-ray film cassette

Light-tight film cassette for exposing blots

  •  Convenient - quickly load and unload with simple push-button latch
  • Light weight - quality light-weight aluminum construction
  • Proven performance - no light leaks even with overnight exposures
WesternBright ECL Spray
WesternBright ECL Spray

Elevate your Western blot results with our chemiluminescent substrate, backed by over 2000 citations for its exceptional sensitivity and up to 10X less antibody usage. Our user-friendly spray device simplifies your workflow – no pipetting, measuring, or mixing needed.

  • Convenience – Just point and spray. No pipetting, measuring, or mixing required
  • High sensitivity – Detect low pg protein amount with film or digital imaging
  • Reduce waste – Eliminate use of tips and tubes for substrate mixing
  • Easy-to-use – Both components of ECL substrate in one convenient spray device

 

Includes:
WesternBright ECL Luminol/enhancer solution
WesternBright Peroxide Chemiluminescent peroxide solution

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