The best choice for routine, film-imaged chemiluminescent Western blots
- Stronger signal than Amersham™ ECL or Pierce™ ECL
- Optimized – for x-ray film imaging
- Sensitive – detect low pg protein amounts
- Lasting signal – up to 6 hrs
- Save money – use up to 10x less antibody
Includes:
WesternBright ECL Luminol/enhancer solution
WesternBright Peroxide Chemiluminescent peroxide solution
The most sensitive chemiluminescent substrate
- Most sensitive of Advansta's HRP substrates, for low-abundance proteins
- Long-lasting signal for flexible imaging
- Low background for high signal-to-noise
- Image by CCD or film
Includes:
WesternBright™ Sirius™Luminol/enhancer solution
WesternBright™ Peroxide Chemiluminescent Detection Reagent
Convenient containers for staining and washing gels and membranes
- Smooth interior – protects membranes and gels from scratches
- Convenient – multiple sizes (5x7, 6x9, 9x11,10.5x15.5 cm) and designs available for different gels and blots
- Attached lid – to protect experiments from dust or debris that can cause speckles on blot images
- Save – minimize antibody and buffer usage by using appropriately sized containers
- Multi-chamber trays – process 6 mini-blots simultaneously, each chamber requires as little as 3 mL of solution
Signal-enhancing Western blot block solution
for the best chemiluminescent signals
- Enhanced signal – greatly enhances Western blotting signals
- Decreased background – decreased non-specific bands and lowered background
- Sensitivity – get better signals for low protein sample concentrations
- Convenience – Pre-made buffer means you save preparation and optimization time
Quantitative chemiluminescent Western blotting
- Sensitive – detect attomoles of protein per band.
- Quantitative – linear range of signal with respect to protein amount exceeds 3 orders of magnitude
- Versatile – Optimized for CCD imaging, and compatible with film detection. Chemifluorescent emissions can be detected with fluorescence imaging systems
- Low background – for high signal to noise ratio
- Long lasting signal – image blots hours after substrate incubation
Includes:
WesternBright Quantum luminol/enhancer solution
WesternBright Quantum stabilized peroxide solution
Transfer sponge pads for more reproducible Western blot transfers
- Resist compression - maintain proper pressure in transfer sandwiches without adding extra blotting papers
- Retain thickness - can be reused over multiple experiments
- Convenient - two sizes (8 x 11 cm and 9 x 15 cm) compatible with a variety of transfer apparatuses
- Standardized - uniform flat construction ensures reproducible and consistent transfers
- Four pads per pack - a full set for common transfer apparatus
High sensitivity X-ray film
for chemiluminescent and other blots
- Performance - excellent images when used with WesternBright ECL
- Value - sensitivity equal to more expensive films
- Flexibility - detect radioisotopes in addition to chemiluminescence
Transparent plastic supports for handling and imaging blots
- Clear images – avoid the wrinkling or bunching that can happen with plastic wrap
- Suitable for all blots – development folders are suitable for chemiluminescence and fluorescence imaging
- Safe blot handling – move your blot without scratching or putting pressure on the surface
- Convenient size – image up to 2 blots simultaneously in one folder
Stripping buffer for stripping antibodies from chemiluminescent Western blots
- Conserve valuable samples - probe the same blot multiple times
- Fast protocol - blot stripped and ready for re-probing in 25 minutes
- Gentle protocol - antibodies removed without harsh reagents or elevated temperatures
- Save time - no need to re-run gels and perform duplicate blots
- Versatile - compatible with both nitrocellulose and PVDF membranes and with a variety of chemiluminescent detection reagents
Convenient nitrocellulose and PVDF membranes for Western blotting
- Performance – quality controlled for background-free Western blots
- Convenience – membranes available pre-cut and as rolls
- High signal-to-noise ratio – low background signal for clearer results
- Flexibility – choose the right membrane for your Western blotting application
Membrane Options:
WesternBright PVDF-FL: fluorescent detection
WesternBright PVDF-CL: chemiluminescent detection; best stability for stripping and re-probing chemiluminescent blots
PVDF membranes available as rolls or pre-cut (7x9 cm, 10x15 cm, or 13x18 cm)
WesternBright NC: all-purpose nitrocellulose membrane
Nitrocellulose membranes available as rolls or pre-cut (8x10 cm)
A chemiluminescent marker for writing on membranes
- Improve accuracy - transform your visible protein ladder standard into a chemiluminescent standard on your Western blot
- Annotate - permanently mark your blots with the date or blot ID
- Compatible - with both X-ray film and CCD imagers
- Confirm HRP substrate stability - the ChemiPen reagent glows when incubated with an HRP substrate, so it can be used to test substrate stability
HRP-conjugated secondary antibodies
for chemiluminescent immunodetection
- Performance – excellent signal in immunoblotting and ELISA applications
- Convenience – each antibody is demonstrated to provide optimal results with Advansta's protein detection systems
- Purity – each antibody is affinity purified to maximize specificity
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