Advansta fluorescent western blot detection and western blotting...

Fluorescent Western blotting

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AdvanStain Iris

Upgrade your protein staining! Boost sensitivity,
no-destaining, and high-contrast.

Detection limit – 2 ng vs Ponceau at 150 ng
Quantitative – effective for lower protein concentrations
No destaining – allows direct chemiluminescent or fluorescent Western blotting without the need for destaining
Versatile – compatible with both nitrocellulose and PVDF membranes

Rapid fluorescent stain for total protein normalization

Convenient – simple protocol that takes less than 20 minutes, no de-staining required
Versatile – compatible with chemiluminescent and IR700/IR800 fluorescent Western blotting
Accurate – linear across a broad range from 1-50μg of lysate, ideal for total protein normalization

FLASHBlot-SD transfer buffer

Rapid high efficiency semi-dry transfer buffer

Fast - high ionic strength formulation allows for protein transfer in 3 to 10 minutes when used with a compatible high current semi-dry system
Compatible - use your existing high current semi-dry transfer apparatus
Reproducible - consistent transfer across entire blot
Versatile - use nitrocellulose or PVDF membranes to achieve transfers with low background and high sensitivity with both chemiluminescent and fluorescent Western blots

Enhance sensitivity of your fluorescent Western blots

Improves sensitivity– Easily visualize faint bands
Compatible – Use with PVDF or Nitrocellulose membranes
Convenient – Ready-to-use solution
Versatile – Enhance signal of both visible and near IR fluorescent Western blots

Fluorescent Western blotting