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Upgrade your protein staining! Boost sensitivity,
no-destaining, and high-contrast.
- Detection limit – 2 ng vs Ponceau at 150 ng
- Quantitative – effective for lower protein concentrations
- No destaining – allows direct chemiluminescent or fluorescent Western blotting without the need for destaining
- Versatile – compatible with both nitrocellulose and PVDF membranes
Rapid fluorescent stain for total protein normalization
- Convenient – simple protocol that takes less than 20 minutes, no de-staining required
- Versatile – compatible with chemiluminescent and IR700/IR800 fluorescent Western blotting
- Accurate – linear across a broad range from 1-50μg of lysate, ideal for total protein normalization
Rapid, high sensitivity chemiluminescent Western blot kit for protein detection
- Rapid – complete Western blot in 1 hour
- Reproducible – fewer hands-on manipulations equate to consistent data
- Convenient – contains all critical reagents required to perform a chemiluminescent Western
- Versatile – kits are available for both mouse and rabbit primary antibodies
Provides high sensitivity and the lowest background
- Enhanced signal – greatly enhances Western blotting signals
- Decreased background – decreased non-specific bands and lowered background
- Sensitivity – get better signals for low protein sample concentrations
- Convenience – Pre-made buffer means you save preparation and optimization time
Ready-to-use wet transfer buffer
Designed for faster, more efficient wet protein transfer with increased sensitivity
FLASHBlot is not your average wet transfer buffer.
- Faster than traditional wet buffers: better results in 20 minutes
- More sensitive detection for low abundance proteins
- Better detection of post-translationally modified proteins
- Better transfer of ALL proteins, including high molecular weight proteins
- Conveniently suitable for any wet transfer set-up
“ We were pleasantly surprised to see that with FLASHBlot we observed the same transferring levels at 25 min than with the lengthier transfer protocol… including for the 300 kDa protein. This reagent will shorten considerably our experimental time. ” - Assistant Professor, UCSF
Rapid high efficiency semi-dry transfer buffer
- Fast - high ionic strength formulation allows for protein transfer in 3 to 10 minutes when used with a compatible high current semi-dry system
- Compatible - use your existing high current semi-dry transfer apparatus
- Reproducible - consistent transfer across entire blot
- Versatile - use nitrocellulose or PVDF membranes to achieve transfers with low background and high sensitivity with both chemiluminescent and fluorescent Western blots
Designed specifically for blot handling and imaging, these clear plastic folders eliminate the frustrations of traditional wrap while improving consistency and clarity
- Wrinkle-free, dust-free imaging – Avoid the wrinkling and dust that cause artifacts in imaging
- Safe handling – No pressure damage or scratching of the membrane
- Suitable for all blots – Image chemiluminescent and fluorescent blots
- Convenient storage – Easily image and store dust-free, up to two blots in each clear folder
Advanced HRP substrate for brighter Westerns
- Optimized – For shorter exposure times
- High sensitivity – Detect low pg protein amount with film or digital imaging
- Long-lasting signal – Capture multiple exposures without worrying about signal decay
- Save money – Use up to 10X less antibody
Includes:
WesternBright ECL Luminol/enhancer solution
WesternBright Peroxide Chemiluminescent peroxide solution
Elevate your Western blot results with our chemiluminescent substrate, backed by over 2000 citations for its exceptional sensitivity and up to 10X less antibody usage. Our user-friendly spray device simplifies your workflow – no pipetting, measuring, or mixing needed.
- Convenience – Just point and spray. No pipetting, measuring, or mixing required
- High sensitivity – Detect low pg protein amount with film or digital imaging
- Reduce waste – Eliminate use of tips and tubes for substrate mixing
- Easy-to-use – Both components of ECL substrate in one convenient spray device
Includes:
WesternBright ECL Luminol/enhancer solution
WesternBright Peroxide Chemiluminescent peroxide solution
Westernbright Quantum was chemically engineered for digital detection to maximize the data from each blot
- High sensitivity – Detect very low amounts of protein down to femtogram levels
- Ultra-low background – Faint bands are clearly seen without high general background
- Long-lasting signal – Capture multiple exposures without worrying about signal decay
- Linear signal over broad dynamic range – Low and high abundance proteins can be imaged simultaneously with a range spanning over three orders of magnitude
Includes:
WesternBright Quantum luminol/enhancer solution
WesternBright Quantum stabilized peroxide solution
The most sensitive chemiluminescent substrate
- Most sensitive of Advansta's HRP substrates, for low-abundance proteins
- Long-lasting signal for flexible imaging
- Low background for high signal-to-noise
- Image by CCD or film
Includes:
WesternBright™ Sirius™Luminol/enhancer solution
WesternBright™ Peroxide Chemiluminescent Detection Reagent
HRP-conjugated secondary antibodies
for chemiluminescent immunodetection
- Performance – excellent signal in immunoblotting and ELISA applications
- Convenience – each antibody is demonstrated to provide optimal results with Advansta's protein detection systems
- Purity – each antibody is affinity purified to maximize specificity
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